Comparison between Values of Low Density Lipoprotein Cholesterol as Estimated by Direct Enzymatic Method with Calculated Methods Applying Friedewald’s Equation and Novel’s Equation: A Cross-sectional Study

Introduction: Lipid profile is routinely used as a screening test to identify the risk of Cardiovascular Diseases (CVD). Elevated Low Density Lipoprotein cholesterol (LDL-c) an important modifiable factor atherosclerotic CVD. The LDL-c lowering strategy known recommendation for prevention and treatment gold standard method estimation β-quantification by ultra centrifugation. Other methods include Direct measurement (D-LDL-c) using enzymatic assay which tedious, time consuming expensive. Hence, calculated Friedewald’s equation (F-LDL-c) in clinical laboratories India. Aim: To compare values estimated direct with obtained Novel’s equation, and, also assess effects both towards stratification Materials Methods: A cross-sectional study, was conducted Central Diagnostic Laboratory Mandya Institute Medical Sciences, Mandya, Karnataka, India, duration three months from July September 2020, where, 600 subjects, aged 20-75 years, visiting routine lipid were included. {F-LDL-c=TC-HDL-c- (TG/5)} {N-LDL-c=TC-HDL-c-(TG/Adjustable factor)}. Values compared D-LDL-c values. at different ranges Total Cholesterol (TC), Triglycerides (TG) High-Density (HDL-c). association between analysed Pearson’s correlation. Receiver Operator Characteristic Curve (ROC) analysis done predict better diagnostic among LDL-c. Results: mean±SD (115.68±36.94 mg/dL) high F-LDL-c (106.95±33.48 N-LDL-c (110.78±32.58 mg/dL). mean difference (4.9±4.36 low (8.75±3.46 Significant positive correlation observed vs (r=0.96; p=<0.001) N-LDLc (r=0.97; p=<0.001). ROC showed maximum AUC value than cut-off 100 mg/dL. led approximately 5% 10% less patients being subjects therapy respectively D-LDL-c. Conclusion: In conclusion, use instead could be associated small net increase agent eligible population primary Replacement would enable improved accuracy especially higher levels TC, TG lower HDL-c.